AtpH 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in WB. Suitable for Arabidopsis thaliana, Nicotiana tabacum, Spinach 和 Thermosynechococcus elongatus.
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Quick Overview for AtpH 抗体 (ABIN249344)
抗原
AtpH
适用
Arabidopsis thaliana, Nicotiana tabacum, Spinach, Thermosynechococcus elongatus
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB)
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特异性
- The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.
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交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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预测反应
- dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 8 kDa (for Arabidopsis thaliana)
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纯化方法
- serum
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免疫原
- purified c subunit from Spinacia oleracea P69447
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anti-AtpH antibody
适用: Arabidopsis thaliana, Chlamydomonas reinhardtii WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1: 1000 - 1: 10 000 with standard ECL (WB)
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说明
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please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- AtpH
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背景
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AGI Code: ATCG00140
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme. -
分子量
- 8 kDa (for Arabidopsis thaliana)
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UniProt
- P69447
抗原
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